11/16/2023 0 Comments Corona commercial 2019![]() ![]() Spend □ TV Impressions □ National Impressions □ Local Impressions □ 2020 doi: 10.1128/ Unlock These Ad Metrics Now National Airings □ First Airing □ Last Airing □ Creatives □ Recently Aired On □ Est. Comparative performance of SARS-CoV-2 detection assays using seven different primer/probe sets and one assay kit. Nalla A.K., Casto A.M., Huang M.W., Perchetti G.A., Sampoleo R., Shrestha L. A contemporary view of coronavirus transcription. Laboratory readiness and response for novel coronavirus (2019-nCoV) in expert laboratories in 30 EU/EEA countries, January 2020. Reusken C.B.E.M., Broberg E.K., Haagmans B., Meijer A., Corman V.M., Papa A. ![]() ![]() Detection of 2019 novel coronavirus (2019-nCoV) by real-time RT-PCR. A pneumonia outbreak associated with a new coronavirus of probable bat origin. Zhou P., Yang X.L., Wang X.G., Hu B., Zhang L., Zhang W. E, envelope protein of SARS-CoV-2 RdRp, RNA-dependent RNA polymerase of SARS-CoV-2 N, nucleocapsid protein of SARS-CoV-2 ORF1ab, open reading frame 1a and b of SARS-CoV-2, includes the RdRp RNA-dependent RNA polymerase of SARS-CoV-2, part of ORF1ab RT-PCR, reverse-transcriptase polymerase chain reaction S, spike protein of SARS-CoV-2 SARS-CoV-2, severe acute respiratory syndrome coronavirus 2. The lines show the mean Ct value for each assay, triangles show the Ct values of the samples with the highest (sample 1) and lowest (sample 10) concentration according to the in-house E-gene PCR. B) Graph depicts only data for those clinical samples (n = 10) with the highest concentration of SARS-CoV-2 RNA and which were positively identified by all RT-PCR assays. For the Seegene kit, one sample was “inconclusive” according to the manufacturer’s guide for interpretation and was therefore counted as “negative”, although a signal was observed for at least one target. 10/13 means 10 out of 13 samples tested positive according to the instructions for data interpretation provided by the manufacturer. The detection rate of the complete RT-PCR kit is indicated below the data points, e.g. Data points above the horizontal dotted line are negative, for plotting purposes indicated with Ct 42.5. A) Graph depicts Ct values obtained for all clinical samples (n = 13) in all RT-PCR assays. RNA isolated from stored SARS-CoV-2-positive clinical samples using the MagNA Pure 96 DNA and Viral NA Small Volume Kit (Roche) was subjected to the various RT-PCR assays according to the manufacturer’s instructions for use, on a LightCycler 480 II (Roche). All rights reserved.ĭifferent RT-PCR kits showed variations in detection rate and Ct values. We conclude that all RT-PCR kits assessed in this study may be used for routine diagnostics of COVID-19 in patients by experienced molecular diagnostic laboratories.ĬOVID-19 Coronavirus In vitro diagnostics RT-PCR SARS-CoV-2 nCoV-2019.Ĭopyright © 2020 The Authors. Importantly, none of the assays showed cross-reactivity with other respiratory (corona)viruses, except as expected for the SARS-CoV-1 E-gene. ![]() Using clinical samples, we observed some variations in detection rate between kits. PCR efficiency was ≥96 % for all assays and the estimated LOD95 varied within a 6-fold range. Finally, we used clinical samples positive for non-coronavirus respiratory viral infections (n = 6) and a panel of RNA from related human coronaviruses to evaluate assay specificity. Furthermore, we ran a panel of SARS-CoV-2-positive clinical samples (n = 13) for a preliminary evaluation of clinical sensitivity. We used serial dilutions of viral RNA to establish PCR efficiency and estimate the 95 % limit of detection (LOD95). The aim of this study was to compare basic analytical and clinical performance of selected RT-PCR kits from seven different manufacturers (Altona Diagnostics, BGI, CerTest Biotec, KH Medical, PrimerDesign, R-Biopharm AG, and Seegene). Many commercial kits have recently become available, but their performance has not yet been independently assessed. real-time reverse transcriptase PCR (RT-PCR). Measures taken to reduce its spread critically depend on timely and accurate identification of virus-infected individuals by the most sensitive and specific method available, i.e. Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has since spread across the globe and is posing a major burden on society. The final months of 2019 witnessed the emergence of a novel coronavirus in the human population. ![]()
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